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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA5-24607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLA2R1 Monoclonal Antibody (CL0474)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EEKTWHEALR SCQADNSALI DITSLAEVEF LVTLLGDENA SETWIGLSSN KIPVSFEWSN DSSVIFTNWH TLEPHIFPNR SQLCVSAEQS EGHWKVKNCE ERLFYICKKA GHVLSDAESG CQEGWERHGG FCYKID Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%. Binds to an epitope located within the peptide sequence KIPVSFEWSN as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0474
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLA2R1 in human kidney tissue lysates using a PLA2R1 Monoclonal Antibody (CL0474).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of PLA2R1 in HeLa cells using the PLA2R1 Monoclonal Antibody (CL0474) (Product # MA5-24607), showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of PLA2R1 in MCF7 cells using the PLA2R1 Monoclonal Antibody (CL0474) (Product # MA5-24607), showing only weak staining in cytosol. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLA2R1 in human kidney (idiopathic membranous nephropathy) using PLA2R1 Monoclonal Antibody (CL0474) (Product # MA5-24607) shows strong membranous positivity in cells in glomeruli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLA2R1 in human kidney using PLA2R1 Monoclonal Antibody (CL0474) (Product # MA5-24607) shows weak membranous positivity in cells in glomeruli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLA2R1 in human pancreas using PLA2R1 Monoclonal Antibody (CL0474) (Product # MA5-24607) shows no positivity in exocrine glandular cells as expected.
