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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-24608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLA2R1 Monoclonal Antibody (CL0485)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EEKTWHEALR SCQADNSALI DITSLAEVEF LVTLLGDENA SETWIGLSSN KIPVSFEWSN DSSVIFTNWH TLEPHIFPNR SQLCVSAEQS EGHWKVKNCE ERLFYICKKA GHVLSDAESG CQEGWERHGG FCYKID Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%. Binds to an epitope located within the peptide sequence VKNCEERLFYICKKA as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0485
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLA2R1 in Lane1: Marker (kDa), Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: PLA2R1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423477). Samples were probed using a PLA2R1 Monoclonal Antibody (Product # MA5-24608).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLA2R1 in human kidney tissuewith membranous nephropathy shows strong membranous immunoreactivity in renal glomeruli. Samples were probed using a PLA2R1 Monoclonal Antibody (Product # MA5-24608).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLA2R1 in normal human kidney tissue shows absence of positivity (negative control). Samples were probed using a PLA2R1 Monoclonal Antibody (Product # MA5-24608).
