HPA012657

antibody from Atlas Antibodies

Targeting: PLA2R1 CLEC13C, PLA2-R, PLA2G1R, PLA2IR

Provider product page for HPA012657

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: HPA012657 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA012657, RRID:AB_2299684
Product name: Anti-PLA2R1
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human PLA2R1, Gene description: phospholipase A2 receptor 1, 180kDa, Alternative Gene Names: CLEC13C, PLA2-R, PLA2G1R, PLA2IR, Validated applications: WB, IHC, Uniprot ID: Q13018, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

PLA2R1 protein structure - HPA012657 shown in red.

Submitted references Target Antigens of Membranous Nephropathy With Syphilis Infection

Zhang L, Zhang M, Xie Q, Wang Y, Ni Z, Liu S, Zhou W
Kidney International Reports 2024;9(2):401-409

Combined evaluation of glomerular phospholipase A2 receptor and immunoglobulin G subclass in membranous nephropathy.

Ueki K, Tsuchimoto A, Matsukuma Y, Ataka E, Okamoto H, Tanaka S, Masutani K, Kitazono T, Nakano T
Clinical kidney journal 2024 Jun;17(6):sfae104

Measurement of urinary exosomal phospholipase A2 receptor is a sensitive method for diagnosis of PLA2R-associated membranous nephropathy.

Wang B, Fu YQ, Xie LJ, Cao JY, Yang M, Li M, Chen TL, Zhang XL, Luo Q, Lv LL, Liu BC
Clinical kidney journal 2024 Jan;17(1):sfad191

Clinicopathologic Features of Antibrush Border Antibody Disease.

Murphy JD, Caza TN, Cassol CA, Storey A, Ambruzs JM, Boils C, Walker PD, Sharma S, Messias N, Hennigar R, Andeen NK, VanBeek C, Palmer M, Sankar L, Sanghi P, Dinesh K, Dicker L, Urisman A, Larsen CP
Kidney international reports 2024 Feb;9(2):370-382

Segmental Membranous Glomerulopathy in Adults

Zhu S, Liu X, Yue S, Luo B, Song Z, Xu X, Wang L, Hou X, Li K, Liang Q, Zhou Z, Chen W, Zheng L
Kidney Diseases 2023;9(6):507-516

C5b-9 Glomerular Deposits Are Associated With Poor Renal Survival in Membranous Nephropathy

Teisseyre M, Beyze A, Perrochia H, Szwarc I, Bourgeois A, Champion C, Chenine L, Serre J, Broner J, Aglae C, Pernin V, Le Quintrec M
Kidney International Reports 2023;8(1):103-114

Single-Nephron GFR in Different Glomerular Basement Membrane Stages of Membranous Nephropathy

Okabayashi Y, Tsuboi N, Marumoto H, Sasaki T, Haruhara K, Kanzaki G, Koike K, Ueda H, Shimizu A, Puelles V, D'Agati V, Yokoo T
Kidney360 2023;4(6):e777-e786

Clinical and Histopathologic Characteristics of Pediatric Patients With Primary Membranous Nephropathy.

Kouri AM, Caza TN, Beck LH Jr, Misurac JM, Evans MD, Phillips CL, Eadon MT, Larsen CP, Andreoli SP, Bu L, Rheault MN, Khalid M
Kidney international reports 2023 Nov;8(11):2368-2375

Complete remission of primary membranous nephropathy following hepatitis E infection.

Takanohashi S, Sugiura T, Koyano A, Ueno T, Rachi H, Shiratori K, Shimasaki M, Igarashi H, Kitayama Y, Togawa A
CEN case reports 2023 Nov;12(4):384-389

Discovery of seven novel putative antigens in membranous nephropathy and membranous lupus nephritis identified by mass spectrometry.

Caza TN, Storey AJ, Hassen SI, Herzog C, Edmondson RD, Arthur JM, Kenan DJ, Larsen CP
Kidney international 2023 Mar;103(3):593-606

Anti-phospholipase A2 receptor antibodies directly induced podocyte damage in vitro

Li Y, Yu J, Wang M, Cui Z, Zhao M
Renal Failure 2022;44(1):304-313

Prevalence of neural epidermal growth factor-like 1- and exostosin 1/exostosin 2-associated membranous nephropathy: a single-center retrospective study in Japan.

Iwakura T, Ema C, Isobe S, Fujikura T, Ohashi N, Kato A, Yasuda H
Scientific reports 2022 Feb 22;12(1):2967

NELL1 is a target antigen in malignancy-associated membranous nephropathy

Caza T, Hassen S, Dvanajscak Z, Kuperman M, Edmondson R, Herzog C, Storey A, Arthur J, Cossey L, Sharma S, Kenan D, Larsen C
Kidney International 2021;99(4):967-976

Qualitative and Quantitative Dosage of the Anti M-Type Phospholipase A2 Receptor Autoantibody: One-Year Experience in Quebec’s Reference Center

Leclerc S, Benkirane K, Nadeau-Fredette A, Elftouh N, Lafrance J, Pichette V, Royal V, Lamarche C, Laurin L
Canadian Journal of Kidney Health and Disease 2021;8

Recurrent membranous nephropathy with a possible alteration in the etiology: a case report

Matsumoto A, Matsui I, Mano K, Mizuno H, Katsuma Y, Yasuda S, Shimada K, Inoue K, Oki T, Hanai T, Kojima K, Kaneko T, Isaka Y
BMC Nephrology 2021;22(1)

Serine Protease HTRA1 as a Novel Target Antigen in Primary Membranous Nephropathy.

Al-Rabadi LF, Caza T, Trivin-Avillach C, Rodan AR, Andeen N, Hayashi N, Williams B, Revelo MP, Clayton F, Abraham J, Lin E, Liou W, Zou CJ, Ramkumar N, Cummins T, Wilkey DW, Kawalit I, Herzog C, Storey A, Edmondson R, Sjoberg R, Yang T, Chien J, Merchant M, Arthur J, Klein J, Larsen C, Beck LH Jr
Journal of the American Society of Nephrology : JASN 2021 Jul;32(7):1666-1681

Neural cell adhesion molecule 1 is a novel autoantigen in membranous lupus nephritis.

Caza TN, Hassen SI, Kuperman M, Sharma SG, Dvanajscak Z, Arthur J, Edmondson R, Storey A, Herzog C, Kenan DJ, Larsen CP
Kidney international 2021 Jul;100(1):171-181

Transforming Growth Factor Beta Receptor 3 (TGFBR3)-Associated Membranous Nephropathy.

Caza TN, Hassen SI, Kenan DJ, Storey A, Arthur JM, Herzog C, Edmondson RD, Bourne TD, Beck LH Jr, Larsen CP
Kidney360 2021 Aug 26;2(8):1275-1286

Membranous nephropathy in patients with HIV: a report of 11 cases

Charu V, Andeen N, Walavalkar V, Lapasia J, Kim J, Lin A, Sibley R, Higgins J, Troxell M, Kambham N
BMC Nephrology 2020;21(1)

Renal Manifestations of Common Variable Immunodeficiency.

Caza TN, Hassen SI, Larsen CP
Kidney360 2020 Jun 25;1(6):491-500

Ultrasensitive Quantitation of Anti-Phospholipase A2 Receptor Antibody as A Diagnostic and Prognostic Indicator of Idiopathic Membranous Nephropathy

Zhang Q, Huang B, Liu X, Liu B, Zhang Y, Zhang Z, Hua J, Fan Y, Hu L, Meng M, Wu M, Wang L, Hu Z, Sun Z
Scientific Reports 2017;7(1)

Response to immunosuppressive therapy in PLA2R- associated and non-PLA2R- associated idiopathic membranous nephropathy: a retrospective, multicenter cohort study

Wang J, Xie Q, Sun Z, Xu N, Li Y, Wang L, Liu S, Xue J, Hao C
BMC Nephrology 2017;18(1)

The PLA2R1-JAK2 pathway upregulates ERRα and its mitochondrial program to exert tumor-suppressive action

Griveau A, Devailly G, Eberst L, Navaratnam N, Le Calvé B, Ferrand M, Faull P, Augert A, Dante R, Vanacker J, Vindrieux D, Bernard D
Oncogene 2016;35(38):5033-5042

Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease

Dauvergne M, Moktefi A, Rabant M, Vigneau C, Kofman T, Burtey S, Corpechot C, Stehlé T, Desvaux D, Rioux-Leclercq N, Rouvier P, Knebelmann B, Boffa J, Frouget T, Daugas E, Jablonski M, Dahan K, Brocheriou I, Remy P, Grimbert P, Lang P, Chazouilleres O, Sahali D, Audard V
Medicine 2015;94(30):e1243

Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy.

Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H
PloS one 2015;10(9):e0138841

Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy

Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H, Remuzzi G
PLOS ONE 2015 September;10(9)

Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy.

Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S
Clinical and experimental nephrology 2015 Aug;19(4):653-60

PLA2R1 kills cancer cells by inducing mitochondrial stress

Augert A, Vindrieux D, Girard C, Le Calvé B, Gras B, Ferrand M, Bouchet B, Puisieux A, de Launoit Y, Simonnet H, Lambeau G, Bernard D
Free Radical Biology and Medicine 2013;65

Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy

Svobodova B, Honsova E, Ronco P, Tesar V, Debiec H
Nephrology Dialysis Transplantation 2013;28(7):1839-1844

Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy

Hoxha E, Kneißler U, Stege G, Zahner G, Thiele I, Panzer U, Harendza S, Helmchen U, Stahl R
Kidney International 2012;82(7):797-804

PLA2R Autoantibodies and PLA2R Glomerular Deposits in Membranous Nephropathy

Debiec H, Ronco P
New England Journal of Medicine 2011;364(7):689-690

Autoantibodies Specific for the Phospholipase A2 Receptor in Recurrent and De Novo Membranous Nephropathy

Debiec H, Martin L, Jouanneau C, Dautin G, Mesnard L, Rondeau E, Mousson C, Ronco P
American Journal of Transplantation 2011;11(10):2144-2152

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423477).
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human kidney and pancreas tissues using HPA012657 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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