HPA012657

PLA2R1 antibody from Atlas Antibodies

CLEC13C, PLA2-R, PLA2G1R, PLA2IR

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA012657

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Antibody Data

Product number

HPA012657

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012657, RRID:AB_1235189

Product name

Anti-PLA2R1

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EEKTWHEALRSCQADNSALIDITSLAEVEFLVTLL
GDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWH
TLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFY
ICKKAGHVLSDAESGCQEGWERHGGFCYKID


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy
Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H, Remuzzi G
PLOS ONE 2015 September;10(9)

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Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy
Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S
Clinical and Experimental Nephrology 2015 August;19(4):653-660

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Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease
Dauvergne M, Moktefi A, Rabant M, Vigneau C, Kofman T, Burtey S, Corpechot C, Stehlé T, Desvaux D, Rioux-Leclercq N, Rouvier P, Knebelmann B, Boffa J, Frouget T, Daugas E, Jablonski M, Dahan K, Brocheriou I, Remy P, Grimbert P, Lang P, Chazouilleres O, Sahali D, Audard V
Medicine 2015 ;94(30)

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Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy
Svobodova B, Honsova E, Ronco P, Tesar V, Debiec H
Nephrology Dialysis Transplantation 2013 July;28(7):1839-1844

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PLA2R1 kills cancer cells by inducing mitochondrial stress
Augert A, Vindrieux D, Girard C, Le Calvé B, Gras B, Ferrand M, Bouchet B, Puisieux A, de Launoit Y, Simonnet H, Lambeau G, Bernard D
Free Radical Biology and Medicine 2013 December;65

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Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy
Hoxha E, Kneißler U, Stege G, Zahner G, Thiele I, Panzer U, Harendza S, Helmchen U, Stahl R
Kidney International 2012 October;82(7):797-804

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Autoantibodies Specific for the Phospholipase A2 Receptor in Recurrent and De Novo Membranous Nephropathy
Debiec H, Martin L, Jouanneau C, Dautin G, Mesnard L, Rondeau E, Mousson C, Ronco P
American Journal of Transplantation 2011 October;11(10):2144-2152

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PLA R Autoantibodies and PLA R Glomerular Deposits in Membranous Nephropathy
Debiec H, Ronco P
New England Journal of Medicine 2011 February;364(7):689-690

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human kidney tissue.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of normal human kidney shows weak membranous positivity in cells in glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows weak membranous positivity in cells in glomeruli.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and pancreas tissues using HPA012657 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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