PA5-44359

antibody from Invitrogen Antibodies

Targeting: ATP6V0D2 ATP6D2, FLJ38708, VMA6

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-44359

Provider

Invitrogen Antibodies

Product name

ATP6V0D2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: GLRLLAQAED FDQMKNVADH YGVYKPLFEA VGGSGGKTLE DVFYEREVQM Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Osteoclast fusion and bone loss are restricted by interferon inducible guanylate binding proteins.

Place DE, Malireddi RKS, Kim J, Vogel P, Yamamoto M, Kanneganti TD
Nature communications 2021 Jan 21;12(1):496

---

A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxia.

Yan K, Wu C, Ye Y, Li L, Wang X, He W, Ren S, Xu Y
Cell proliferation 2020 Mar;53(3):e12778

---

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Montoro DT, Haber AL, Biton M, Vinarsky V, Lin B, Birket SE, Yuan F, Chen S, Leung HM, Villoria J, Rogel N, Burgin G, Tsankov AM, Waghray A, Slyper M, Waldman J, Nguyen L, Dionne D, Rozenblatt-Rosen O, Tata PR, Mou H, Shivaraju M, Bihler H, Mense M, Tearney GJ, Rowe SM, Engelhardt JF, Regev A, Rajagopal J
Nature 2018 Aug;560(7718):319-324

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 A20 inhibits osteoclastogenesis through downregulating autophagy under hypoxia. A, After becoming pre-osteoclasts, BMMCs were cultured in CM from cells transfected with lentivirus A20 for overexpression. Osteoclast differentiation was measured by TRAP staining. B, The cytoskeleton of multinuclear cells was detected by IF (bar = 100 mum). C, The areas of hydroxyapatite resorption were measured by toluidine blue staining (bar = 100 mum). D, The expression of osteoclast-related factors (CTSK, MMP9, V-ATPase D2 and TRAP) in BMMCs was detected by Western blot. Data are presented as mean +- SD, ** P < .01, *** P < .001

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 A20 inhibits osteoclastogenesis through downregulating autophagy under hypoxia. A, After becoming pre-osteoclasts, BMMCs were cultured in CM from cells transfected with lentivirus A20 for silencing. Osteoclast differentiation was measured by TRAP staining. B, The cytoskeleton of multinuclear cells was detected by IF (bar = 100 mum). C, The areas of hydroxyapatite resorption were measured by toluidine blue staining (bar = 100 mum). D, The expression of osteoclast-related factors (CTSK, MMP9, V-ATPase D2 and TRAP) in BMMCs was detected by Western blot. Data are presented as mean +- SD, * P < .05, ** P < .01, *** P < .001