PA5-98618

antibody from Invitrogen Antibodies

Targeting: ATP6V0D2 ATP6D2, FLJ38708, VMA6

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-98618

Provider

Invitrogen Antibodies

Product name

ATP6V0D2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

4.045 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP6V0D2 in HepG2 cells using a ATP6V0D2 polyclonal antibody (Product # PA5-98618) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ATP6V0D2 in paraffin embedded human kidney tissue using a ATP6V0D2 polyclonal antibody (Product # PA5-98618) at a dilution of 1:500. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.