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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00011059-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00011059-M01, RRID:AB_509107
- Product name
- WWP1 monoclonal antibody (M01), clone 1A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant WWP1.
- Antigen sequence
CSSSPTIEIQENGDALHENGEPSARTTARLAVEGT
NGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVA
ARPKNTPAPKPLASEPADDTVNGESSSFAPTDNAS
VTGT- Isotype
- IgG
- Antibody clone number
- 1A7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
Functional Characterization of a WWP1/Tiul1 Tumor-derived Mutant Reveals a Paradigm of Its Constitutive Activation in Human Cancer.
WWP2-WWP1 ubiquitin ligase complex coordinated by PPM1G maintains the balance between cellular p73 and ΔNp73 levels.
Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53.
WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) delays cellular senescence by promoting p27(Kip1) degradation in human diploid fibroblasts.
The E3 ubiquitin ligase WWP1 regulates ΔNp63-dependent transcription through Lys63 linkages.
Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.
Basheer WA, Harris BS, Mentrup HL, Abreha M, Thames EL, Lea JB, Swing DA, Copeland NG, Jenkins NA, Price RL, Matesic LE
Journal of molecular and cellular cardiology 2015 Nov;88:1-13
Journal of molecular and cellular cardiology 2015 Nov;88:1-13
Functional Characterization of a WWP1/Tiul1 Tumor-derived Mutant Reveals a Paradigm of Its Constitutive Activation in Human Cancer.
Courivaud T, Ferrand N, Elkhattouti A, Kumar S, Levy L, Ferrigno O, Atfi A, Prunier C
The Journal of biological chemistry 2015 Aug 21;290(34):21007-18
The Journal of biological chemistry 2015 Aug 21;290(34):21007-18
WWP2-WWP1 ubiquitin ligase complex coordinated by PPM1G maintains the balance between cellular p73 and ΔNp73 levels.
Chaudhary N, Maddika S
Molecular and cellular biology 2014 Oct 1;34(19):3754-64
Molecular and cellular biology 2014 Oct 1;34(19):3754-64
Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53.
Cheng Q, Cao X, Yuan F, Li G, Tong T
Biochemical and biophysical research communications 2014 Jun 6;448(3):248-54
Biochemical and biophysical research communications 2014 Jun 6;448(3):248-54
WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) delays cellular senescence by promoting p27(Kip1) degradation in human diploid fibroblasts.
Cao X, Xue L, Han L, Ma L, Chen T, Tong T
The Journal of biological chemistry 2011 Sep 23;286(38):33447-56
The Journal of biological chemistry 2011 Sep 23;286(38):33447-56
The E3 ubiquitin ligase WWP1 regulates ΔNp63-dependent transcription through Lys63 linkages.
Peschiaroli A, Scialpi F, Bernassola F, El Sherbini el S, Melino G
Biochemical and biophysical research communications 2010 Nov 12;402(2):425-30
Biochemical and biophysical research communications 2010 Nov 12;402(2):425-30
Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.
Edwards TL, Clowes VE, Tsang HT, Connell JW, Sanderson CM, Luzio JP, Reid E
The Biochemical journal 2009 Sep 14;423(1):31-9
The Biochemical journal 2009 Sep 14;423(1):31-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01), clone 1A7.Lane 1: WWP1 transfected lysate(105.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged WWP1 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of WWP1 transfected lysate using anti-WWP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with WWP1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
