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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-58728 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF18A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LHCHHLHLQN KDLKAQIRHM MDLACLQEQQ HRQTEAVLNA LLPTLRKQYC TLKEAGLSNA AFESDFKEIE HLVERKKVVV WADQTAEQPK QND
- Concentration
- 0.28 mg/mL
Submitted references The Proliferation of Glioblastoma Is Contributed to Kinesin Family Member 18A and Medical Data Analysis of GBM.
Wang LB, Zhang XB, Liu J, Liu QJ
Frontiers in genetics 2022;13:858882
Frontiers in genetics 2022;13:858882
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KIF18A in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a KIF18A Polyclonal Antibody (Product # PA5-58728).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of KIF18A in human cell line U-251 MG shows positivity in cytoplasm & microtubules. Samples were probed using a KIF18A Polyclonal Antibody (Product # PA5-58728).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of KIF18A in human cell line U-2 OS using a KIF18A Polyclonal Antibody (Product # PA5-58728) shows localization to cytosol, microtubules and cytokinetic bridge.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIF18A in human rectal tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a KIF18A Polyclonal Antibody (Product # PA5-58728).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 KIF18A expression in human GBM tissues. (A) Expression levels of KIF18A in human GBM and normal tissues (GBM: glioblastoma, * p < 0.05). (B) Immunohistochemical (IHC) staining of KIF18A protein in human GBM tissues (x100 and x200 magnification, resp.). (C) IHC staining of KIF18A in the adjacent tissues (x100 and x200 magnification, resp. ).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Knockdown of KIF18A impaired the tumor growth of GBM cells in mice. (A) U87 cells infected with KIF18A or shRNA control plasma were repopulated into nude mice. After 2 weeks, tumors were drained and tumor size was measured weekly ( n = three groups). Tumor growth curves estimated three tumors. (B) Analysis shows that KIF18A is expressed in control or non-KIF18A tumor tissue from mice. (C) IHC analysis shows that Ki67 is expressed in control tissues or tissues lacking KIF18A. Results are expressed as overall standard deviation, * p < 0.05.
