PAB23531

antibody from Abnova Corporation

Targeting: KIF18A DKFZP434G2226, PPP1R99

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23531

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23531, RRID:AB_11121743

Product name

KIF18A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KIF18A.

Antigen sequence

LHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTE
AVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIE
HLVERKKVVVWADQTAEQPKQND


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with KIF18A polyclonal antibody (Cat # PAB23531) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with KIF18A polyclonal antibody (Cat # PAB23531) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with KIF18A polyclonal antibody (Cat # PAB23531) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)