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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 19245-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF18A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSVTEEDLC HHMKVVVRVR PENTKEKAAG FHKVVHVVDK HILVFDPKQE EVSFFHGKKT TNQNVIKKQN KDLKFVFDAV FDETSTQSEV FEHTTKPILR SFLNGYNCTV LAYGATGAGK THTMLGSADE PGVMYLTMLH LYKCMDEIKE EKICSTAVSY LEVYNEQIRD LLVNSGPLAV REDTQKGVVV HGLTLHQPKS SEEILHLLDN GNKNRTQHPT DMNATSSRSH AVFQIYLRQQ DKTASINQNV RIAKMSLIDL AGSERASTSG AKGTRFVEGT (1-279 aa encoded by BC048347)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 19245-1-AP (KIF18A antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 19245-1-AP (KIF18A antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-KIF18A (IP:19245-1-AP, 4ug; Detection:19245-1-AP 1:500) with Jurkat cells lysate 2400ug.
