AM32005PU-N

antibody from OriGene

Targeting: PTPRR EC-PTP, PCPTP1, PTP-SL, PTPBR7, PTPRQ

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM32005PU-N

Provider

OriGene

Product name

Ptprr mouse monoclonal antibody, clone 6A6, Purified

Antibody type

Monoclonal

Description

Ptprr mouse monoclonal antibody, clone 6A6, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

Ptprr

Isotype

IgG

Antibody clone number

6A6

Vial size

100 µg

Concentration

0.5 mg/ml (BSA concentration = 1%)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 2: Monoclonal antibody 6A6 immunoprecipitates from different brain regions, visualized on blot using STEP absorbed a-SL that is immunoreactive towards all PTPRR proteins.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 1: Neuro-2a cells were transiently transfected with PTP-SL or STEP expression plasmids, as indicated above the lanes. Mock transfected cells were included as negative controls. Lysates were directly subjected to Western blot analysis using monoclonal antibodies 6A6. In the PTP-SL Lane: at 60 kDa PTP-SL, at 42 and 37 kDa two PTPPBS? isoforms.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 3: Immunolocalization of PTPRR protein in Mouse brain. Brain cryosections were stained using a mixture of three different monoclonal antibodies (1E3, 3E11 and 6A6) immunoreactive towards the common part in PTPRR isoforms and that cross-react with STEP. Positive staining is observed in the Purkinje cells of the cerebellum (A), both the neurones and neuropil of the striatum (B). The staining of the striatum reflects STEP immunoreactivity.

Validation comment

IHC