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- Antigen structure
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- Product number
- PA5-19009 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The suppressive effects of miR-508-5p on the odontogenic differentiation of human dental pulp stem cells by targeting glycoprotein non-metastatic melanomal protein B.
Liu F, Wang X, Yang Y, Hu R, Wang W, Wang Y
Stem cell research & therapy 2019 Jan 22;10(1):35
Stem cell research & therapy 2019 Jan 22;10(1):35
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 miR-508-5p inhibits the odontogenic differentiation of hDPCs. hDPCs were transfected with negative control (NC), negative control of siRNA (NC-Si), miR-508-5p mimics, or miR-508-5p siRNA, respectively. a On day 7 of odontogenic differentiation, the expression levels of the odontogenic marker genes ( ALP , DMP-1 , DSPP , and OCN ) were detected by qRT-PCR. b The expression levels of the odontogenic marker genes ( ALP , DMP-1 , DSPP , and OCN ) were detected by Western blotting on day 7 of odontogenic differentiation. c The Western blot results of GPNMB protein were quantified. d ALP activity decreased in the miR-508-5p mimics group and increased in the miR-508-5p siRNA on day 7 of odontogenic induction. e On day 14 after treatment, Alizarin Red S staining was performed to show the inhibitory effects of miR-508-5p mimics on the matrix mineralization of hDPCs. * p < 0.05
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 GPNMB lacking 3'-UTR can rescue the effect of miR-508-5p. a The expression levels of the odontogenic marker genes ( ALP , DMP-1 , DSPP , and OCN ) were detected by qRT-PCR in each indicated group. b The expression levels of the odontogenic marker genes ( ALP , DMP-1 , DSPP , and OCN ) were detected by Western blotting in each indicated group. c The Western blot results of GPNMB protein were quantified. d On day 7 after the co-transfections of miR-508-5p mimics and GPNMB lacking 3'-UTR of hDPCs, ALP activity increased significantly. e On day 14 after treatment, Alizarin Red S staining was performed to show the effects on the matrix mineralization of hDPCs in each indicated group. * p < 0.05
