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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F54949 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Insulin receptor substrate 2 Antibody / IRS2
- Antibody type
- Polyclonal
- Description
- This highly specific Insulin receptor substrate 2 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Insulin receptor substrate 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HEK293 cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with Insulin receptor substrate 2 antibody (green) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human hepatocellular carcinoma tissue with Insulin receptor substrate 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
