AF7069

antibody from Novus Biologicals

Targeting: DGKE DAGK6, DGK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7069

Provider

Novus Biologicals

Product name

Sheep Polyclonal DGK-epsilon Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects recombinant human DGK-epsilon in direct ELISAs. Detects human and mouse DGK-epsilon in Western blots.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse DGK-epsilon by Western Blot. Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DGK-epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DGK-epsilon at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

DGK-epsilon in Human Brainstem. DGK-epsilon was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human DGK-epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.