AP2740b

antibody from Abgent

Targeting: SEMA6A HT018, KIAA1368, SEMA, SEMA6A1, SEMAQ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2740b

Provider

Abgent

Proper citation

Abgent Cat#AP2740b, RRID:AB_2285733

Product name

SEMA6A Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of SEMA6A Antibody (C-term) Pab (Cat. #AP2740b) pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. SEMA6A (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-SEMA6A Antibody (C-term)(Cat.#AP2740b) pre-incubated with(right lane) and without(left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human brain tissue reacted with SEMA6A antibody (C-term) (Cat.#AP2740b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50