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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5921 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Semaphorin 6A Antibody / SEMA6A
- Antibody type
- Polyclonal
- Description
- This highly specific Semaphorin 6A antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Semaphorin 6A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEK293, 2) HeLa, 3) Raji, 4) U-2 OS, 5) Jurkat, 6) Caco-2, 7) rat brain, 8) rat PC-12, 9) mouse brain and 10) mouse RAW264.7 with Semaphorin 6A antibody. Predicted molecular weight ~114 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with Semaphorin 6A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with Semaphorin 6A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA6A antibody.
