Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-77379 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CNTFR (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Chemokine CCL5 promotes robust optic nerve regeneration and mediates many of the effects of CNTF gene therapy.
Xie L, Yin Y, Benowitz L
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar 2;118(9)
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar 2;118(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates with CNTFR (extracellular) polyclonal antibody (Product # PA5-77379) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates with CNTFR (extracellular) polyclonal antibody (Product # PA5-77379) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CNTFR (extracellular) in rat hippocampus. A) Samples were probed with CNTFR (extracellular) polyclonal antibody (Product # PA5-77379) using a dilution of 1:400, and incubated with DAPI. B) Nuclei stained image. C) Merged image of Panels A and B.