ACR-051-50UL

antibody from Invitrogen Antibodies

Targeting: CNTFR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ACR-051-50UL

Provider

Invitrogen Antibodies

Product name

CNTFR alpha (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-CNTFR alpha (extracellular) Antibody (#ACR-051), (1:200).4-6. Anti-CNTFR alpha (extracellular) Antibody , preincubated with CNTFR alpha (extracellular) Blocking Peptide (#BLP-CR051).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-CNTFR alpha (extracellular) Antibody (#ACR-051), (1:200).4-6. Anti-CNTFR alpha (extracellular) Antibody , preincubated with CNTFR alpha (extracellular) Blocking Peptide (#BLP-CR051).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CNTF receptor alpha in rat hippocampus - Immunohistochemical staining of rat hippocampal CA1 region using Anti-CNTFR alpha (extracellular) Antibody (#ACR-051), (1:400). A. CNTFR alpha staining (green) appears in astrocytes in the CA1 region (arrows). B. Nuclei staining using DAPI as the counterstain (green). C. Merged image of panels A and B.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection ofCNTF receptor alpha by indirect flow cytometry in live intact mouse BV-2 microglia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-CNTFR alpha (extracellular)  Antibody   (#ACR-051), (5μg) + goat- Anti-rabbit-FITC.