Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-65220 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65220, RRID:AB_964821
- Product name
- Mouse Monoclonal Histone H1 Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. This antibody recognises an antigen found in the nucleus. Antibody also preferentially reacts with histone H1/ DNA nucleosome as shown by ELISA and western blotting. In WB using non-reduced samples it recognises primarily a 30-32kDa histone H1 band, but other bands are present as well. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells. The epitope has not been mapped.
- Reactivity
- Human, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220] - FFPE pig heart stained with NB100-65220 at 1:500.