19649-1-AP

antibody from Proteintech Group

Targeting: H1-2 H1.2, H1c, H1F2, H1s-1, HIST1H1C

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

19649-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#19649-1-AP, RRID:AB_10694432

Product name

Histone H1.2 antibody

Antibody type

Polyclonal

Description

Histone H1.2 antibody (Cat. #19649-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression.

Murray-Nerger LA, Lozano C, Burton EM, Liao Y, Ungerleider NA, Guo R, Gewurz BE
Nature communications 2024 May 16;15(1):4156

---

Targeted inhibition of DHODH is synergistic with BCL2 blockade in HGBCL with concurrent MYC and BCL2 rearrangement.

Liu L, Mo W, Chen M, Qu Y, Wang P, Liang Y, Yan X
BMC cancer 2024 Jun 25;24(1):761

---

Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1.

Hu X, Zhu Z, Liu K, Liu J, Li J, Wang Z
Translational cancer research 2024 Jan 31;13(1):102-111

---

Single-cell RNA sequencing of cervical exfoliated cells reveals potential biomarkers and cellular pathogenesis in cervical carcinogenesis.

Sheng B, Pan S, Ye M, Liu H, Zhang J, Zhao B, Ji H, Zhu X
Cell death & disease 2024 Feb 12;15(2):130

---

Downregulation of lncRNA APCDD1L-AS1 due to DNA hypermethylation and loss of VHL protein expression promotes the progression of clear cell renal cell carcinoma.

Yang W, Zhou J, Zhang Z, Zhang K, Xu Y, Li L, Cai L, Gong Y, Gong K
International journal of biological sciences 2022;18(6):2583-2596

---

Histone functions as a cell-surface receptor for AGEs.

Itakura M, Yamaguchi K, Kitazawa R, Lim SY, Anan Y, Yoshitake J, Shibata T, Negishi L, Sugawa H, Nagai R, Uchida K
Nature communications 2022 May 27;13(1):2974

---

Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.

Wang Z, Liang M, Liu B, Yang L
Food science and biotechnology 2022 Jun;31(6):745-758

---

L-Methionine inhibits 4-hydroxy-2-nonenal accumulation and suppresses inflammation in growing rats.

Wang Z, Liang M, Li H, Liu B, Yang L
Nutrition research and practice 2022 Dec;16(6):729-744

---

l-Arginine prevents 4-hydroxy-2-nonenal accumulation and depresses inflammation via inhibiting NF-κB activation.

Liang M, Wang Z, Li H, Liu B, Yang L
Journal of biochemical and molecular toxicology 2022 Aug;36(8):e23087

---

Rice protein suppresses 4-hydroxy-2-nonenal-induced inflammation owing to methionine availability.

Wang Z, Liang M, Li H, Liu B, Yang L
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2022 Aug 1;47(8):826-838

---

The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.

Tian J, Wang Y, Ding M, Zhang Y, Chi J, Wang T, Jiao B, Jian Z, Yi X, Huang Y, Liu L, Li K, Chen J, Wang G, Gao T, Li C, Li Q
Oxidative medicine and cellular longevity 2021;2021:7617839

---

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Lao M, Zhang X, Ma T, Xu J, Yang H, Duan Y, Ying H, Zhang X, Guo C, Qiu J, Bai X, Liang T
Oncogene 2021 Apr;40(17):3136-3151

---

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Wang Z, Cai L, Li H, Liang M, Zhang Y, Wu Q, Yang L
Journal of food biochemistry 2020 May;44(5):e13180

---

Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.

Li H, Liang M, Wang Z, Zhang Y, Wu Q, Yang L
Journal of medicinal food 2020 Jun;23(6):565-574

---

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.

Wang Q, Zhang Q, Gan Z, Li H, Yang Y, Zhang Y, Zhao X
Reproduction in domestic animals = Zuchthygiene 2020 Feb;55(2):189-199

---

RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.

Guo Z, Tian Y, Guo Y, Li B, Liu X, Xie K, Song Y, Wang D
Frontiers in cellular neuroscience 2019;13:392

---

Evidence for a novel mechanism of influenza A virus host adaptation modulated by PB2-627.

Liu X, Yang C, Sun X, Lin X, Zhao L, Chen H, Jin M
The FEBS journal 2019 Sep;286(17):3389-3400

---

Rice protein reduces DNA damage by activating the p53 pathway and stimulating endogenous antioxidant response in growing and adult rats.

Liang M, Li H, Wang Z, Cai L, Yang L
Journal of the science of food and agriculture 2019 Oct;99(13):6097-6107

---

Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway.

Wang Z, Liang M, Li H, Cai L, Yang L
International journal of molecular sciences 2019 Dec 6;20(24)

---

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Wang Z, Liang M, Li H, Cai L, He H, Wu Q, Yang L
Journal of the science of food and agriculture 2019 Aug 15;99(10):4849-4862

---

New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis.

Liu WT, Lv YJ, Yang RC, Fu JY, Liu L, Wang H, Cao Q, Tan C, Chen HC, Wang XR
Journal of neuroinflammation 2018 Oct 19;15(1):291

---

l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.

Liang M, Wang Z, Li H, Cai L, Pan J, He H, Wu Q, Tang Y, Ma J, Yang L
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 May;115:315-328

---

Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

Ito-Ishida A, Yamalanchili HK, Shao Y, Baker SA, Heckman LD, Lavery LA, Kim JY, Lombardi LM, Sun Y, Liu Z, Zoghbi HY
Nature neuroscience 2018 Jun;21(6):794-798

---

A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy.

Yang C, Zang W, Tang Z, Ji Y, Xu R, Yang Y, Luo A, Hu B, Zhang Z, Liu Z, Zheng X
Cancer research 2018 Feb 15;78(4):1069-1082

---

HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3.

Liu X, Yang C, Hu Y, Lei E, Lin X, Zhao L, Zou Z, Zhang A, Zhou H, Chen H, Qian P, Jin M
Frontiers in immunology 2017;8:350

---

C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis.

Wang Y, Yue D, Xiao M, Qi C, Chen Y, Sun D, Zhang N, Chen R
Journal of proteome research 2015 Feb 6;14(2):804-13

---

The linker histone h1.2 is an intermediate in the apoptotic response to cytokine deprivation in T-effectors.

Garg M, Perumalsamy LR, Shivashankar GV, Sarin A
International journal of cell biology 2014;2014:674753

---

PP2A inhibitors suppress migration and growth of PANC-1 pancreatic cancer cells through inhibition on the Wnt/β-catenin pathway by phosphorylation and degradation of β-catenin.

Wu MY, Xie X, Xu ZK, Xie L, Chen Z, Shou LM, Gong FR, Xie YF, Li W, Tao M
Oncology reports 2014 Aug;32(2):513-22

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP(HIST1H1C antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using HIST1H1C antibody 19649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.

Sample type

cell line