PA5-112452

antibody from Invitrogen Antibodies

Targeting: H1-2 H1.2, H1c, H1F2, H1s-1, HIST1H1C

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112452

Provider

Invitrogen Antibodies

Product name

Di-Methyl-Histone H1.2 (Lys45) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.14 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Methyl Histone H1.2 (Lys45) in multiple sample types using a Methyl Histone H1.2 (Lys45) polyclonal antibody (Product # PA5-112452) at a dilution of 1:2000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:40,000. Sample types that were tested include: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Methyl Histone H1.2 (Lys45) in multiple sample types using a Methyl Histone H1.2 (Lys45) polyclonal antibody (Product # PA5-112452) at a dilution of 1:2000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:40,000. Sample types that were tested include: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate .

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Methyl Histone H1.2 (Lys45) in Hela cells using a Polyclonal antibody (Product # PA5-112452) at a dilution of 1:10. Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Methyl Histone H1.2 (Lys45) in Hela cells using a Polyclonal antibody (Product # PA5-112452) at a dilution of 1:10. Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation analysis of Methyl Histone H1.2 (Lys45) in 4*10^6 Hela cells using a Methyl Histone H1.2 (Lys45) polyclonal antibody (Product #PA5-112452). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg anti-HIST1H1C or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Methyl Histone H1.2 (Lys45) in Hela whole cell lysate using a Polyclonal antibody (Product #PA5-112452). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 1: Rabbit control IgG, Lane 2: 5 µg of primary antibody and 1mg of Hela whole cell lysate, Lane 3: 20 µg of Hela whole cell lysate.