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LearnPA5-112457
antibody from Invitrogen Antibodies
Targeting: H1-2
H1.2, H1c, H1F2, H1s-1, HIST1H1C
Western blot
ELISA Immunocytochemistry
Immunoprecipitation Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-112457 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acetyl-Histone H1.2 (Lys96) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Methyl Histone H1.2 (Lys186) in multiple sample types using a Methyl Histone H1.2 (Lys186) polyclonal antibody (Product # PA5-112456) at a dilution of 1:500. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:40,000. Sample types that were tested include: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Polyclonal antibody (Product # PA5-112457) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457) at a dilution of 1:100. The cells were treated with 30mM sodium butyrate for 4 hours. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation Assay of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457). 4 x 10^6 Hela cells were treated with 30 mM sodium butyrate for 4h, treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg of the primary anibody or a control of normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457).
