PA5-112457

antibody from Invitrogen Antibodies

Targeting: H1-2 H1.2, H1c, H1F2, H1s-1, HIST1H1C

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112457

Provider

Invitrogen Antibodies

Product name

Acetyl-Histone H1.2 (Lys96) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.29 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Polyclonal antibody (Product # PA5-112457) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457) at a dilution of 1:100. The cells were treated with 30mM sodium butyrate for 4 hours. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Polyclonal antibody (Product # PA5-112457) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457) at a dilution of 1:100. The cells were treated with 30mM sodium butyrate for 4 hours. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation Assay of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457). 4 x 10^6 Hela cells were treated with 30 mM sodium butyrate for 4h, treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg of the primary anibody or a control of normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation Assay of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457). 4 x 10^6 Hela cells were treated with 30 mM sodium butyrate for 4h, treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg of the primary anibody or a control of normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Acetyl Histone H1.2 (Lys96) in Hela cells using a Acetyl Histone H1.2 (Lys96) polyclonal antibody (Product # PA5-112457).