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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-32009 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H1.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa nucleus.
- Concentration
- 0.88 mg/mL
Submitted references Linker histone H1.2 and H1.4 affect the neutrophil lineage determination.
Sollberger G, Streeck R, Apel F, Caffrey BE, Skoultchi AI, Zychlinsky A
eLife 2020 May 11;9
eLife 2020 May 11;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Histone H1.2 Polyclonal Antibody (Product # PA5-32009). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HeLa nucleus. 12% SDS PAGE. Histone H1.2 Polyclonal Antibody (Product # PA5-32009) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Histone H1.2 in paraformaldehyde-fixed HeLa cells using a Histone H1.2 polyclonal antibody (Product # PA5-32009) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Histone H1.2 (Product # PA5-32009) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
