Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00064221-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064221-M02, RRID:AB_1713130
- Product name
- ROBO3 monoclonal antibody (M02), clone 2H1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ROBO3.
- Antigen sequence
MTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPL
PPPEEEASWALELRAAGSMSSLERERSGERKAVQA
VPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCS
TAGSNSSRGSSSSRGSRGPGRSRSQSRSQSQRPGQ
KRREEPR- Isotype
- IgG
- Antibody clone number
- 2H1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ROBO3 expression in transfected 293T cell line by ROBO3 monoclonal antibody (M02), clone 2H1.Lane 1: ROBO3 transfected lysate (Predicted MW: 16.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ROBO3 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol