- PA1-661 - Invitrogen Antibodies DNM2 antibody

Submitted references Identification of host proteins differentially associated with HIV-1 RNA splice variants.

Knoener R, Evans E 3rd, Becker JT, Scalf M, Benner B, Sherer NM, Smith LM
eLife 2021 Feb 25;10

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots.

Ressnerova A, Novotny F, Michalkova H, Pumera M, Adam V, Heger Z
ACS nano 2021 Aug 24;15(8):12899-12910

The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina.

Toulis V, García-Monclús S, de la Peña-Ramírez C, Arenas-Galnares R, Abril JF, Todi SV, Khan N, Garanto A, Costa MDC, Marfany G
Cell reports 2020 Nov 10;33(6):108360

JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by β-Arrestin.

Mayberry CL, Soucy AN, Lajoie CR, DuShane JK, Maginnis MS
Journal of virology 2019 Apr 15;93(8)

SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2.

Søreng K, Munson MJ, Lamb CA, Bjørndal GT, Pankiv S, Carlsson SR, Tooze SA, Simonsen A
EMBO reports 2018 Apr;19(4)

Small molecule induced oligomerization, clustering and clathrin-independent endocytosis of the dopamine transporter.

Sorkina T, Ma S, Larsen MB, Watkins SC, Sorkin A
eLife 2018 Apr 9;7

Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells.

Bohmer N, Jordan A
Beilstein journal of nanotechnology 2015;6:167-76

Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.

Olofsson A, Nygård Skalman L, Obi I, Lundmark R, Arnqvist A
mBio 2014 May 20;5(3):e00979-14

Protein partners of dynamin-1 in the retina.

Grossman GH, Ebke LA, Beight CD, Jang GF, Crabb JW, Hagstrom SA
Visual neuroscience 2013 Jul;30(4):129-39

Induction of the alternative NF-κB pathway by lymphotoxin αβ (LTαβ) relies on internalization of LTβ receptor.

Ganeff C, Remouchamps C, Boutaffala L, Benezech C, Galopin G, Vandepaer S, Bouillenne F, Ormenese S, Chariot A, Schneider P, Caamaño J, Piette J, Dejardin E
Molecular and cellular biology 2011 Nov;31(21):4319-34

Dynamin 2 is required for the enhancement of HIV-1 infectivity by Nef.

Pizzato M, Helander A, Popova E, Calistri A, Zamborlini A, Palù G, Göttlinger HG
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 17;104(16):6812-7

Beta-amyloid-induced dynamin 1 depletion in hippocampal neurons. A potential mechanism for early cognitive decline in Alzheimer disease.

Kelly BL, Vassar R, Ferreira A
The Journal of biological chemistry 2005 Sep 9;280(36):31746-53

Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin.

Hale ML, Marvaud JC, Popoff MR, Stiles BG
Infection and immunity 2004 Apr;72(4):2186-93

Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin.

Hale ML, Marvaud JC, Popoff MR, Stiles BG
Infection and immunity 2004 Apr;72(4):2186-93

Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference.

Huang F, Khvorova A, Marshall W, Sorkin A
The Journal of biological chemistry 2004 Apr 16;279(16):16657-61

Sorting nexin 9 participates in clathrin-mediated endocytosis through interactions with the core components.

Lundmark R, Carlsson SR
The Journal of biological chemistry 2003 Nov 21;278(47):46772-81

Aquaporin-2 localization in clathrin-coated pits: inhibition of endocytosis by dominant-negative dynamin.

Sun TX, Van Hoek A, Huang Y, Bouley R, McLaughlin M, Brown D
American journal of physiology. Renal physiology 2002 Jun;282(6):F998-1011

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti- Dynamin II Rabbit polyclonal Antibody (Product # PA1-661, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A band ~ 98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by overnight transfer method. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody (Product # PA1-661) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody (Product # PA1-661) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG 5 Cells depleted of AP-2 and dynamin II show reduced uptake of H. pylori vesicles. (A) H. pylori vesicles were incubated with siRNA-transfected AGS cells for 20 min. In comparison to vesicle uptake in AGS cells transfected with negative siRNA (100%), vesicle internalization was affected as follows in the knockdowns: AP-2, 63%; dynamin II, 80%; AP-2 plus dynamin II, 46%. In comparison to Tfn uptake in AGS cells transfected with negative siRNA (100%), Tfn internalization was affected as follows in the knockdowns: AP-2, 35%; dynamin II, 58%; AP-2 plus dynamin II, 29%. All experiments were performed in triplicate ( n = 3). Statistical significance was as follows: with AP-2, P for Ves was 0.0039 and P for Tfn was 0.0039; for dynamin II, P for Ves was 0.0039 and P for Tfn was 0.0039); for AP-2 plus dynamin II, P for Ves was 0.0039 and P for Tfn was 0.0039). Ves, vesicles; Tfn, transferrin. (B) Western blot analysis to measure knockdown efficiency in AGS cells. Knockdown efficiency was as follows: for AP-2 (AP2M1) (m2 subunit), 81%; for dynamin II (DNM2), 84%; for AP-2 (m2 subunit) plus dynamin II, 89% and 91%. Tubulin or aldolase was used as the loading control to correlate the amount of protein in each sample.

PA1-661

Antibody data