PA5-19800

antibody from Invitrogen Antibodies

Targeting: DNM2 CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19800

Provider

Invitrogen Antibodies

Product name

Dynamin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Heat mediated antigen retrieval recommended prior to tissue staining. This antibody is predicted to react with cow, Xenopus laevis and zebrafish based on sequence homology.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.8 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.

Fiuza M, Rostosky CM, Parkinson GT, Bygrave AM, Halemani N, Baptista M, Milosevic I, Hanley JG
The Journal of cell biology 2017 Oct 2;216(10):3323-3338

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Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.

Fajardo VA, Smith IC, Bombardier E, Chambers PJ, Quadrilatero J, Tupling AR
Brain and behavior 2016 Jun;6(6):e00470

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Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.

Fajardo VA, Bombardier E, McMillan E, Tran K, Wadsworth BJ, Gamu D, Hopf A, Vigna C, Smith IC, Bellissimo C, Michel RN, Tarnopolsky MA, Quadrilatero J, Tupling AR
Disease models & mechanisms 2015 Aug 1;8(8):999-1009

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Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.

Windheim M, Hansen B
Cellular signalling 2014 Jan;26(1):49-55

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using Product # PA5-19800, anti-Dynamin 2 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. PICK1 binds dynamin via BAR and GTPase domains and regulates dynamin polymerization. (A) Endogenous PICK1 interacts with endogenous dynamin 1, 2, and 3 in neurons. Extracts of cortical neurons were immunoprecipitated with PICK1 antibody or control IgG. Proteins were detected by Western blotting. Input is 5% of offered protein. Blots shown are representative of more than five independent experiments. (B) PICK1 interacts directly with dynamin. GST or GST-PICK1 was immobilized on glutathione agarose and incubated with purified HA-dynamin. Proteins were detected by Western blotting. Blots shown are representative of four independent experiments. (C) PICK1 interacts with the GTPase domain of dynamin. HEK293 cells were cotransfected with plasmids expressing Flag-PICK1 and GFP, GFP-dynamin 2, or dynamin domains as indicated. Cells were lysed and incubated with GFP-trap agarose. Blots shown are representative of five independent experiments. (D) Dynamin interacts with the PICK1 BAR domain. HEK cells were cotransfected with plasmids expressing HA-dynamin and GFP, GFP-PICK1, or truncations, as indicated in the top panel. Cells were lysed and incubated with GFP-trap agarose. Blots shown are representative of five independent experiments. (E) PICK1 promotes dynamin polymerization. His 6 -PICK1 at the concentrations indicated was incubated with HA-dynamin. Polymerized dynamin was pelleted by centrifugation, and protein in the supernatant and pellet was analyzed by Western bl