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LearnPA5-29017
antibody from Invitrogen Antibodies
Targeting: DNM2
CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29017 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dynamin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Molt-4, Raji. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.44 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Dynamin 2 Polyclonal Antibody (Product # PA5-29017). Sample (30 µg whole cell lysate). A: MOLT4. B: Raji. 7.5% SDS PAGE. Dynamin 2 Polyclonal Antibody (Product # PA5-29017) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Dynamin 2 Polyclonal Antibody (Product # PA5-29017) and a 110kDa band corresponding to Dynamin 2 was observed across all cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), HEK-293 (Lane 3), K-562 (Lane 4), Raji (Lane 5) and Mouse Lung (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Dynamin 2 Polyclonal Antibody (Product # PA5-29017) and a 110kDa band corresponding to Dynamin 2 was observed across all tissues tested along with certain uncharacterized bands at ~35, ~55 kDa. Tissue extracts (50 µg lysate) of Rat Brain (Lane 1), Mouse Brain (Lane 2) and Mouse Kidney (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Dynamin 2 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Dynamin 2 polyclonal antibody (Product # PA5-29017) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dynamin 2 Polyclonal Antibody detects DNM2 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNM2 protein stained by Dynamin 2 Polyclonal Antibody (Product # PA5-29017) diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope].
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynamin 2 (Product # PA5-29017) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
