Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP6683C - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP6683c, RRID:AB_10551864
- Product name
- WNT4 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Estimated diversity of messenger RNAs in each murine spermatozoa and their potential function during early zygotic development.
Fang P, Zeng P, Wang Z, Liu M, Xu W, Dai J, Zhao X, Zhang D, Liang D, Chen X, Shi S, Zhang M, Wang L, Qiao Z, Shi H
Biology of reproduction 2014 May;90(5):94
Biology of reproduction 2014 May;90(5):94
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of WNT4 Antibody (Center) (Cat. #AP6683c) in mouse bladder tissue lysates (35ug/lane).WNT4 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human bladder carcinoma reacted with WNT4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB468 cells using WNT4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50