AP6683b

antibody from Abcepta

Targeting: WNT4 WNT-4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6683b

Provider

Abcepta

Proper citation

Abgent Cat#AP6683b, RRID:AB_1968419

Product name

WNT4 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

β-catenin is a central mediator of pro-fibrotic Wnt signaling in systemic sclerosis.

Beyer C, Schramm A, Akhmetshina A, Dees C, Kireva T, Gelse K, Sonnylal S, de Crombrugghe B, Taketo MM, Distler O, Schett G, Distler JH
Annals of the rheumatic diseases 2012 May;71(5):761-7

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of WNT4 antibody (C-term) (Cat. #AP6683b) in 293 cell line lysates (35ug/lane). WNT4 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using WNT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis."

Primary Ab dilution

1:50~100