Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX101085 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101085, RRID:AB_2038248
- Product name
- Wnt4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/β-Catenin Signaling Pathway in Diabetic Rats.
Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model.
Bai L, Huo B, Chen Z, Guo Q, Xu J, Fang J, Zhang J, Zhang F
Evidence-based complementary and alternative medicine : eCAM 2017;2017:3054145
Evidence-based complementary and alternative medicine : eCAM 2017;2017:3054145
Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model.
Xu Y, Lei H, Guan R, Gao Z, Li H, Wang L, Song W, Gao B, Xin Z
Biochemical and biophysical research communications 2014 Jul 18;450(1):87-92
Biochemical and biophysical research communications 2014 Jul 18;450(1):87-92
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Non-transfected 293T lysates B: WNT4 transfected 293T lysates 10% SDS PAGE GTX101085 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT4 antibody detects WNT4 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (GTX101085) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT4 antibody detects WNT4 protein by western blot analysis. Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody (GTX101085) at a dilution of 1:2500.The V5 was used as internal control (GTX117997, 1:2500) shown at the bottom panel. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using WNT4(GTX101085) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT4 antibody detects WNT4 protein at secreted on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WNT4 antibody (GTX101085) dilution: 1:500.