MA5-50344

antibody from Invitrogen Antibodies

Targeting: WNT4 WNT-4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50344

Provider

Invitrogen Antibodies

Product name

WNT4 Recombinant Rabbit Monoclonal Antibody (HL1889)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

HL1889

Vial size

100 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNT4 using WNT4 Recombinant Monoclonal Antibody (Product # MA5-50344) in Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) at a dilution of 1:5,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNT4 using WNT4 Recombinant Monoclonal Antibody (Product # MA5-50344) in Whole zebrafish extract (30 μg) at a dilution of 1:1,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNT4 using WNT4 Recombinant Monoclonal Antibody (Product # MA5-50344) in Non-transfected (–) and transfected (+) 293T whole cell extracts at a dilution of 1:10,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of WNT4 using WNT4 Recombinant Monoclonal Antibody (Product # MA5-50344) in HeLa cells at a dilution of 1:100. Cells were fixed in ice-cold MeOH for 5 min. Green: Wnt4 stained by Wnt4 antibody (MA5-50344) diluted at 1:100. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of WNT4 using WNT4 Recombinant Monoclonal Antibody (Product # MA5-50344) in Paraffin embedded mouse kidney tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.