PA5-117789

antibody from Invitrogen Antibodies

Targeting: APOL2 APOL-II

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117789

Provider

Invitrogen Antibodies

Product name

Apolipoprotein L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Apolipoprotein L2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Apolipoprotein L2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Apolipoprotein L2 in Lane A: 0.5 mg U-251MG Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 37 kDa , Observed band size : 40 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of Apolipoprotein L2 in human brain. Samples were incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:500 .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Apolipoprotein L2 in human urinary bladder with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789, 1:500 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of Apolipoprotein L2 in human urinary bladder. Samples were incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:500 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Apolipoprotein L2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Apolipoprotein L2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa.