Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-117789 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apolipoprotein L2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Apolipoprotein L2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Apolipoprotein L2 in Lane A: 0.5 mg U-251MG Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 37 kDa , Observed band size : 40 kDa .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of Apolipoprotein L2 in human brain. Samples were incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:500 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Apolipoprotein L2 in human urinary bladder with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789, 1:500 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of Apolipoprotein L2 in human urinary bladder. Samples were incubated with polyclonal antibody (Product # PA5-117789) with a dilution of 1:500 .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apolipoprotein L2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with Apolipoprotein L2 Polyclonal Antibody (Product # PA5-117789) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Apolipoprotein L2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa.