MA5-28133

antibody from Invitrogen Antibodies

Targeting: TLN2 ILWEQ, KIAA0320

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-28133 - Provider product page
Provider: Invitrogen Antibodies
Product name: Talin Monoclonal Antibody (TA205)
Antibody type: Monoclonal
Antigen: Other
Description: Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a approximately 225 kDa cytoskeletal protein.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: TA205
Vial size: 200 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TLN2 protein structure - MA5-28133 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Talin 1 was performed using MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Talin 1 Goat Polyclonal Antibody (Product # MA5-28133) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing cytoplasmic and ruffled membrane localization of Talin 1 in MDA-MB-231 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Talin 1 was performed using MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Talin 1 Goat Polyclonal Antibody (Product # MA5-28133) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing cytoplasmic and ruffled membrane localization of Talin 1 in MDA-MB-231 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.