Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10518 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10518, RRID:AB_11188064
- Product name
- CARM1 monoclonal antibody, clone 3H2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CARM1.
- Isotype
- IgG
- Antibody clone number
- 3H2
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) against MCF-7 (1), HeLa (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), COS-7 (8), and PC-12 (9) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and ovarian cancer tissues (B) using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of LoVo cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green) and negative control (purple).
- Validation comment
- Flow Cytometry