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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- R31387 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRMT4 Antibody / CARM1
- Antibody type
- Polyclonal
- Description
- This highly specific PRMT4 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PRMT4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of PRMT4 antibody and Lane 1: rat spleen; 2: human placenta; 3: (r) kidney; 4: mouse NIH3T3; 5: (h) HeLa; 6: (h) HL60; 7: (h) Jurkat lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293T, 2) human HeLa, 3) human MCF7, 4) human HepG2, 5) rat testis and 6) rat C6 cell lysate with PRMT4 antibody. Predicted molecular weight ~66 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with PRMT4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human 293T cells with PRMT4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRMT4 antibody.
