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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-421A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for IP at 6 µg per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (A300-421A-2) and rabbit anti-CARM1 antibody A300-420A. For blotting immunoprecipitated CARM1, A300-421A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARM1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Product # A300-421A; lot 4) used at 1:1,000 (1 µg/mL). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for IP at 6 µg per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (lot A300-421A-3) and rabbit anti-CARM1 antibody (Product # A300-420A). For blotting immunoprecipitated CARM1 (Product # A300-421A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
