MA5-15796

antibody from Invitrogen Antibodies

Targeting: CARM1 PRMT4

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15796

Provider

Invitrogen Antibodies

Product name

PRMT4 Monoclonal Antibody (3H2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15796 targets CARM1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, Mouse and Rat samples. The MA5-15796 immunogen is purified recombinant fragment of human CARM1 expressed in E. Coli. MA5-15796 detects CARM1 which has a predicted molecular weight of approximately 65kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3H2

Vial size

100 µL

Concentration

Conc. not determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using CARM1 monoclonal antibody (Product # MA5-15796) (Green). Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PRMT4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRMT4 Monoclonal Antibody (3H2) (Product # MA5-15796) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Lovo cells using CARM1 monoclonal antibody (Product # MA5-15796) (green) and negative control (purple).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous PRMT4 protein at specific gene loci using Anti-PRMT4 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-PRMT4 Monoclonal Antibody (Product # MA5-15796, 8 ul) on sheared chromatin from 2 million HCT 116 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the promoters of CCND1 and PS2 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.