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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-52837 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF149 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LLLHTVKHGE KGIDVDAENC AVCIENFKVK DIIRILPCKH IFHRICIDPW LLDHRTCPMC KLDVIKALGY WGEPGDVQEM PAPESPPGRD PAANLSLALP DDDGSDESSP PSASPAESEP QCDPSFKGDA GENT
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF149 in control (vector only transfected HEK293T lysate) and RNF149 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RNF149 Polyclonal Antibody (Product # PA5-52837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF149 in control (vector only transfected HEK293T lysate) and RNF149 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RNF149 Polyclonal Antibody (Product # PA5-52837).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RNF149 in human cell line U-2 OS shows positivity in plasma membrane & vesicles. Samples were probed using a RNF149 Polyclonal Antibody (Product # PA5-52837).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF149 in human small intestine shows distinct membranous positivity in glandular cells. Samples were probed using a RNF149 Polyclonal Antibody (Product # PA5-52837).
