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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA011424 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA011424, RRID:AB_1079825
- Product name
- Anti-RNF149
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RNF149, Gene description: ring finger protein 149, Alternative Gene Names: FLJ90504, Validated applications: ICC, IHC, WB, Uniprot ID: Q8NC42, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references RNF149 Promotes HCC Progression through Its E3 Ubiquitin Ligase Activity.
Guo Z, Jiang P, Dong Q, Zhang Y, Xu K, Zhai Y, He F, Tian C, Sun A
Cancers 2023 Oct 29;15(21)
Cancers 2023 Oct 29;15(21)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and RNF149 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406531).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
- Sample type
- Human
