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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-407A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Reticulocalbin-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Reticulocalbin-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody (Product # A300-407A; lot A300-407A-3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCN1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCN1 antibody A300-407A (Lot A300-407A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RCN1 was also immunoprecipitated by rabbit anti-RCN1 antibody lot A300-407A-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCN1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 (Product # A300-407A; lot 3) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RCN1 (Cat. No. A300-407A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Reticulocalbin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody (Product # A300-407A; lot A300-407A-3) used for IP at 6 µg per reaction. Reticulocalbin-1 was also immunoprecipitated by a previous lot of this antibody (lot A300-407A-2). For blotting immunoprecipitated Reticulocalbin-1 (Product # A300-407A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
