MA5-23862

antibody from Invitrogen Antibodies

Targeting: CCL27 ALP, CTACK, CTAK, ESkine, ILC, PESKY, SCYA27, skinkine

Western blot (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23862

Provider

Invitrogen Antibodies

Product name

CCL27 Monoclonal Antibody (124308)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

124308

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CCL27 in BaF3 mouse pro‚B cell line transfected with human CCR1. Samples were incubated in CCL27 monoclonal antibody (Product # MA5-23862). Recombinant Human CCL27/CTACK chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL27/CTACK Monoclonal Antibody. The ND50 is typically 0.5-3.0 µg/mL.