LS-C675704

antibody from LSBio

Targeting: EIF3M FLJ29030, GA17, hfl-B5, PCID1, TANGO7

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C675704

Provider

LSBio

Product name

EIF3M / PCID1 Antibody LS-C675704

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes:EIF3M antibody at 2.02 ug/ml Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Mouse gonadal tissue Lane 4: Human placenta tissue Lane 5: Mouse stomach tissue Lane 6: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43,28 kDa Observed band size: 43 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating EIF3M in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating EIF3M in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)