PA5-44000

antibody from Invitrogen Antibodies

Targeting: DCUN1D3 DKFZp686O0290, FLJ41725, MGC48972, SCCRO3

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-44000

Provider

Invitrogen Antibodies

Product name

DCUN1D3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: GHREEQVPPC GKPGGDILVN GTKKAEAATE ACQLPTSSGD AGRESKSNAE Sequence homology: Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Selective inhibition of cullin 3 neddylation through covalent targeting DCN1 protects mice from acetaminophen-induced liver toxicity.

Zhou H, Lu J, Chinnaswamy K, Stuckey JA, Liu L, McEachern D, Yang CY, Bernard D, Shen H, Rui L, Sun Y, Wang S
Nature communications 2021 May 11;12(1):2621

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 DI-1548 and DI-1859 selectively inhibit cullin 3 neddylation in different types of cells. The effects of a dose range of the indicated compound on the protein level in osteosarcoma U2OS ( a ) and in immortalized THLE2 liver cell lines ( b ). The protein levels of neddylated cullin 3, 1, 2, 4A, 4B, and 5 (N-Cul3, 1, 2, 4A, 4B, and 5) and unneddylated cullin 3, 1, 2, 4A, 4B, and 5 (Cul3, 1, 2, 4A, 4B, and 5), as well as the substrates of cullins mediated CRLs, including p21, Bim, Nrf2 and CDT1, and HO-1 were examined by western blotting analysis. GAPDH was used as a loading control. c DI-1548 rapidly and selectively inhibits the neddylation of cullin 3 in osteosarcoma U2OS. The protein levels of neddylated cullin 3, 1 (N-Cul3, 1) and unneddylated cullin 3, 1 (Cul3, 1) were examined by western blotting analysis. GAPDH was used as a loading control. d DI-1548 effectively engages cellular DCN1 protein. U2OS cell line was treated by dose ranges of compounds DI-1548, DI-1548DD, or DI-591 for 1 h, then was heated at 53 degC. The cells were snap frozen in liquid nitrogen and thawed for three cycles. The levels of DCN1 and DCN3 in whole cell lysates were examined by western blotting analysis. GAPDH was used as a loading control. Representative images of two independent experiments are shown for a - d . Source data are provided as a Source Data file.