14-3189-82

antibody from Invitrogen Antibodies

Targeting: CDCP1 CD318, SIMA135

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-3189-82

Provider

Invitrogen Antibodies

Product name

CD318 (CDCP1) Monoclonal Antibody (CSTEM26), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

This monoclonal antibody CSTEM26 reacts with human CD318, also known as CUB domain-containing protein 1 (CDCP1) and Trask. Applications Reported: This CSTEM26 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CSTEM26 antibody has been tested by flow cytometric analysis of normal human induced pluripotent stem cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CSTEM26

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C, do not freeze

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of a mixture of human iPSC and the C2C12 murine cell line with Anti-Human TRA-1-60 PerCP-eFluor 710 (Product # 46-8863-82) and 0.25 µg Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (left) or 0.25 µg of Anti-Human CD318 (CDCP1) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82).