TA502231

antibody from OriGene

Targeting: CDCP1 CD318, SIMA135

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502231

Provider

OriGene

Proper citation

OriGene Cat#TA502231, RRID:AB_11129815

Product name

CDCP1 mouse monoclonal antibody, clone OTI5B3 (formerly 5B3)

Antibody type

Monoclonal

Description

CDCP1 mouse monoclonal antibody, clone OTI5B3 (formerly 5B3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

CDCP1

Isotype

IgG

Antibody clone number

OTI5B3

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDCP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (RC220633, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-CDCP1 antibody(TA502231),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC