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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA5-32447 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX1 Recombinant Rabbit Monoclonal Antibody (JJ20-40)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JJ20-40
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.
Kimura K, Koizumi T, Urasawa T, Ohta Y, Takakura D, Kawasaki N
Scientific reports 2021 May 27;11(1):11169
Scientific reports 2021 May 27;11(1):11169
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SOX1 in SHG-44 cells using a SOX1 Monoclonal antibody (Product # MA5-32447) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SOX1 in SHG-44 cells using a SOX1 Monoclonal antibody (Product # MA5-32447) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SOX1 with paraffin-embedded human brain. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal SOX1 (Product # MA5-32447) at a dilution of 1:200 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SOX1 with paraffin-embedded mouse brain. The tissues were blocked in 5% BSA (30 min, room temp), washed with ddH2O and PBS, incubated with recombinant monoclonal SOX1 (Product # MA5-32447) at a dilution of 1:50 (30 min, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SOX1 with paraffin-embedded rat brain. The tissues were blocked in 5% BSA (30 min, room temp), washed with ddH2O and PBS, incubated with recombinant monoclonal SOX1 (Product # MA5-32447) at a dilution of 1:50 (30 min, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.
