H00001479-M01

antibody from Abnova Corporation

Targeting: CSTF3 CstF-77

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001479-M01

Provider

Abnova Corporation

Proper citation

Product name

CSTF3 monoclonal antibody (M01), clone 1D4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CSTF3.

Antigen sequence

MSGDGATEQAAEYVPEKVKKAEKKLEENPYDLDAW
SILIREAQNQPIDKARKTYERLVAQFPSSGRFWKL
YIEAEVTILFYFFLYQYCSIHCSDRKQVRNIAN


Isotype

IgG

Antibody clone number

1D4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Polyadenylation site-specific differences in the activity of the neuronal βCstF-64 protein in PC-12 cells.

Shankarling GS, MacDonald CC
Gene 2013 Oct 25;529(2):220-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.Lane 1: CSTF3 transfected lysate(11.44 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol