10219-1-AP

antibody from Proteintech Group

Targeting: EIF3D eIF3-p66, eIF3-zeta, EIF3S7

Provider product page for 10219-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [20]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 10219-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10219-1-AP, RRID:AB_2096880
Product name: EIF3D antibody
Antibody type: Polyclonal
Description: EIF3D antibody (Cat. #10219-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, yeast and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Yeast
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EIF3D protein structure - 10219-1-AP shown in red.

Submitted references Branched chemically modified poly(A) tails enhance the translation capacity of mRNA.

Chen H, Liu D, Guo J, Aditham A, Zhou Y, Tian J, Luo S, Ren J, Hsu A, Huang J, Kostas F, Wu M, Liu DR, Wang X
Nature biotechnology 2024 Mar 22;

Potential antidepressant-like effects of N-3 polyunsaturated fatty acids through inhibition of endoplasmic reticulum stress.

Tang M, Liu T, Shen Y, Wang L, Xue Y, Zhao T, Xie K, Gong Z, Yin T
Psychopharmacology 2023 Sep;240(9):1877-1889

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cui Q, Bi H, Lv Z, Wu Q, Hua J, Gu B, Huo C, Tang M, Chen Y, Chen C, Chen S, Zhang X, Wu Z, Lao Z, Sheng N, Shen C, Zhang Y, Wu ZY, Jin Z, Yang P, Liu H, Li J, Bai G
Cell 2023 Feb 16;186(4):803-820.e25

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.

Thompson L, Depledge DP, Burgess HM, Mohr I
Cell reports 2022 May 3;39(5):110767

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, Yamamoto T, Nakagawa M, Yamanaka S, Takahashi K
iScience 2022 May 20;25(5):104289

Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c.

Fujii K, Zhulyn O, Byeon GW, Genuth NR, Kerr CH, Walsh EM, Barna M
Developmental cell 2021 Nov 8;56(21):2928-2937.e9

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.

Yu NK, McClatchy DB, Diedrich JK, Romero S, Choi JH, Martínez-Bartolomé S, Delahunty CM, Muotri AR, Yates JR 3rd
iScience 2021 Nov 19;24(11):103321

G3BP1 interacts with YWHAZ to regulate chemoresistance and predict adjuvant chemotherapy benefit in gastric cancer.

Zhao J, Fu X, Chen H, Min L, Sun J, Yin J, Guo J, Li H, Tang Z, Ruan Y, Wang X, Sun Y, Huang L
British journal of cancer 2021 Jan;124(2):425-436

A translational program that suppresses metabolism to shield the genome.

Balukoff NC, Ho JJD, Theodoridis PR, Wang M, Bokros M, Llanio LM, Krieger JR, Schatz JH, Lee S
Nature communications 2020 Nov 13;11(1):5755

EIF3D promotes sunitinib resistance of renal cell carcinoma by interacting with GRP78 and inhibiting its degradation.

Huang H, Gao Y, Liu A, Yang X, Huang F, Xu L, Danfeng X, Chen L
EBioMedicine 2019 Nov;49:189-201

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Aulas A, Lyons SM, Fay MM, Anderson P, Ivanov P
Cell death & disease 2018 Nov 13;9(11):1129

Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.

Latosinska A, Mokou M, Makridakis M, Mullen W, Zoidakis J, Lygirou V, Frantzi M, Katafigiotis I, Stravodimos K, Hupe MC, Dobrzynski M, Kolch W, Merseburger AS, Mischak H, Roubelakis MG, Vlahou A
Oncotarget 2017 Sep 19;8(41):69435-69455

Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Shi Z, Fujii K, Kovary KM, Genuth NR, Röst HL, Teruel MN, Barna M
Molecular cell 2017 Jul 6;67(1):71-83.e7

A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

Leen EN, Sorgeloos F, Correia S, Chaudhry Y, Cannac F, Pastore C, Xu Y, Graham SC, Matthews SJ, Goodfellow IG, Curry S
PLoS pathogens 2016 Jan;12(1):e1005379

The LIF-mediated molecular signature regulating murine embryo implantation.

Rosario GX, Hondo E, Jeong JW, Mutalif R, Ye X, Yee LX, Stewart CL
Biology of reproduction 2014 Sep;91(3):66

Int6 regulates both proteasomal degradation and translation initiation and is critical for proper formation of acini by human mammary epithelium.

Suo J, Snider SJ, Mills GB, Creighton CJ, Chen AC, Schiff R, Lloyd RE, Chang EC
Oncogene 2011 Feb 10;30(6):724-36

Int6 and Moe1 interact with Cdc48 to regulate ERAD and proper chromosome segregation.

Otero JH, Suo J, Gordon C, Chang EC
Cell cycle (Georgetown, Tex.) 2010 Jan 1;9(1):147-61

A practical approach to isolate 48S complexes: affinity purification and analyses.

Locker N, Lukavsky PJ
Methods in enzymology 2007;429:83-104

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.

Locker N, Easton LE, Lukavsky PJ
The EMBO journal 2007 Feb 7;26(3):795-805

Affinity purification of eukaryotic 48S initiation complexes.

Locker N, Easton LE, Lukavsky PJ
RNA (New York, N.Y.) 2006 Apr;12(4):683-90

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP(EIF3D antibody) at dilution of 1:400
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using EIF3D antibody 10219-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line