H00002189-M01

antibody from Novus Biologicals

Targeting: FANCG FAG, XRCC9

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00002189-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00002189-M01, RRID:AB_538710

Product name

Mouse Monoclonal FANCG Antibody

Antibody type

Monoclonal

Description

IgG purified. FANCG - Fanconi anemia, complementation group G

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks.

Zhu B, Yan K, Li L, Lin M, Zhang S, He Q, Zheng D, Yang H, Shao G
Oncogene 2015 May 28;34(22):2867-78

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Areca nut induces miR-23a and inhibits repair of DNA double-strand breaks by targeting FANCG.

Tsai YS, Lin CS, Chiang SL, Lee CH, Lee KW, Ko YC
Toxicological sciences : an official journal of the Society of Toxicology 2011 Oct;123(2):480-90

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Stimulation of homology-directed repair at I-SceI-induced DNA breaks during the permissive life cycle of human cytomegalovirus.

Kulkarni AS, Fortunato EA
Journal of virology 2011 Jun;85(12):6049-54

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Adipocyte fatty acid binding protein in a Caucasian population: a new marker of metabolic syndrome?

Stejskal D, Karpisek M
European journal of clinical investigation 2006 Sep;36(9):621-5

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Four human FANCG polymorphic variants show normal biological function in hamster CHO cells.

Hinz JM, Nham PB, Yamada NA, Tebbs RS, Salazar EP, Hinz AK, Mohrenweiser HW, Jones IM, Thompson LH
Mutation research 2006 Dec 1;602(1-2):34-42

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG over-expressed 293 cell line, cotransfected with FANCG Validated Chimera RNAi ( Cat # H00002189-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FANCG monoclonal antibody (M01), clone 2C8 (Cat # H00002189-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.Lane 1: FANCG transfected lysate(68.6 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FANCG Antibody (2C8) [H00002189-M01] - FANCG monoclonal antibody (M01), clone 2C8 Analysis of FANCG expression in Hela S3 NE.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01] - Analysis of monoclonal antibody to FANCG on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG transfected lysate using anti-FANCG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FANCG MaxPab rabbit polyclonal antibody.