PA5-27117

antibody from Invitrogen Antibodies

Targeting: FANCG FAG, XRCC9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27117

Provider

Invitrogen Antibodies

Product name

FANCG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, NIH-3T3.

Concentration

1 mg/mL

References

Submitted references

Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome.

Lagundžin D, Hu WF, Law HCH, Krieger KL, Qiao F, Clement EJ, Drincic AT, Nedić O, Naldrett MJ, Alvarez S, Woods NT
PloS one 2019;14(8):e0220568

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FANCG Polyclonal Antibody (Product # PA5-27117). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. FANCG Polyclonal Antibody (Product # PA5-27117) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FANCG Polyclonal Antibody (Product # PA5-27117). Sample (30 µg of whole cell lysate). A: H1299. 7.5% SDS PAGE. FANCG Polyclonal Antibody (Product # PA5-27117) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FANCG in paraformaldehyde-fixed HeLa cells using a FANCG polyclonal antibody (Product # PA5-27117) at a 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 FA core complex assembly and FA protein expression in EndoC-betaH3 cells in response to glucose stimulation. A. Dot Plot of FA proteins identified in the FANCA co-IP analysis between 5 mM and 20 mM glucose conditions in EndoC-betaH3 cells. Dot size represents relative abundance. Dot color indicates the average number of spectra in each experiment. BFDR is represented by the outline shading. B. Expression of a panel of FA proteins in EndoC-betaH3 cells cultured in either 5 mM or 20 mM glucose for 1 hour determined by western blot. C. Confirmation of FANCB knockdown by shRNA (shFANCB) compared to non-targeting scrambled control shRNA (shScr) in EndoC-betaH3 cells. D. GSIS profiles in EndoC-betaH3 shScr or shFANCB transduced cells. Paired two-tailed Student's t-test p -values are displayed in the indicated comparisons above the graph. n = 3, mean +/- SEM, n.s. = not statistically significant. E. Intracellular insulin content of shScr or shFANCB EndoC-betaH3 cells used in the GSIS assays in panel C. Paired two-tailed Student's t-test p -values are displayed in the indicated comparisons above the graph. n = 3, mean +/- SEM.