21215-1-AP

antibody from Proteintech Group

Targeting: ATOH1 bHLHa14, HATH1, MATH-1, Math1

Provider product page for 21215-1-AP

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Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
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Product number: 21215-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21215-1-AP, RRID:AB_10733126
Product name: ATOH1 antibody
Antibody type: Polyclonal
Description: ATOH1 antibody (Cat. #21215-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ATOH1 protein structure - 21215-1-AP shown in red.

Submitted references VGLL4 modulates Paneth cells and sustains intestinal homeostasis.

Zhang H, Wang Z, Wang X, Yu W, Zhang G, Zhang H, Lu Y, Sun Y, Lu T, Li X, Yang R, Sun J, Xu J, Huang S, Ma X, Ren J, Tang N, Cheng Z, Yu J, Wei F, Zhou H, Li J, Qin J, Jin Y, Zhang L
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Activation of IL-17/CEBPB/PFKFB3 triggers energy metabolic switching and goblet cell differentiation in ulcerative colitis.

Zhao H, Hu Z
Biochemical pharmacology 2026 Mar;245:117654

Oral administration of low-molecular-weight heparin ameliorates colitis by enhancing the gut mucus barrier via microbial tryptophan metabolites.

Zheng D, Chen S, Feng H, Zhang S, Zhang C, Wang Y, Tan W, Qing Q, Liu L, Liu X, Wang Z, Liang L, Sun J, Chen Y
British journal of pharmacology 2026 Feb;183(3):560-580

L1TD1 promotes colorectal mucinous adenocarcinoma progression by enhancing ABCC3 mRNA stability.

He H, Yuan J, Wang H, Chen L, Liu B, Cui Z, Xu Y, Zhang G, Yang H
Oncogene 2026 Apr;45(12):1071-1086

Crotonate enhances intestinal regeneration after injury via HBO1-mediated H3K14 crotonylation.

Xiao Y, Yu S, Zhang M, Zhong N, Hua S, Fang Z, Zhang Z, Liu H, Tan R, Liu Y, Chen YG
Nature communications 2025 Oct 2;16(1):8800

Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.

Catozzi A, Peiris Pagès M, Humphrey S, Revill M, Morgan D, Roebuck J, Chen Y, Davies-Williams B, Brennan K, Mukarram Hossain ASM, Makeev VJ, Satia K, Sfyri PP, Galvin M, Coles D, Lallo A, Pearce SP, Kerr A, Priest L, Foy V, Carter M, Caeser R, Chan JM, Rudin CM, Blackhall F, Frese KK, Dive C, Simpson KL
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Lethal co-expression intolerance underlies the mutually exclusive expression of ASCL1 and NEUROD1 in SCLC cells.

Watanabe H, Inoue Y, Tsuchiya K, Asada K, Suzuki M, Ogawa H, Tanahashi M, Watanabe T, Matsuura S, Yasuda K, Ohnishi I, Imokawa S, Yasui H, Karayama M, Suzuki Y, Hozumi H, Furuhashi K, Enomoto N, Fujisawa T, Funai K, Shinmura K, Sugimura H, Inui N, Suda T
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Gut sulfide metabolism modulates behavior and brain bioenergetics.

Kumar R, Sykes DJ, Band VI, Schaller ML, Patel R, Vitvitsky V, Sajjakulnukit P, Singhal R, Wong HKA, Hourigan SK, Ichinose F, Lyssiotis CA, Shah YM, Banerjee R
Proceedings of the National Academy of Sciences of the United States of America 2025 Jun 24;122(25):e2503677122

Metabolic adaptations direct cell fate during tissue regeneration.

Chaves-Perez A, Millman SE, Janaki-Raman S, Ho YJ, Hinterleitner C, Barthet VJA, Morris JP 4th, Barriga FM, Reyes J, Kyaw A, Pasolli HA, Pe'er D, Thompson CB, Finley LWS, Cross JR, Lowe SW
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Sinisan, a compound Chinese herbal medicine, alleviates acute colitis by facilitating colonic secretory cell lineage commitment and mucin production.

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The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs).

Sabatini C, Lin HJ, Ovik G, Hall R, Lee T
BMC molecular and cell biology 2025 Jan 20;26(1):5

Notch1 is Required to Maintain Supporting Cell Identity and Vestibular Function during Maturation of the Mammalian Balance Organs.

Heffer A, Lee C, Mayernik JP, Holt JC, Kiernan AE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2025 Feb 26;45(9)

Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling.

Lehr S, Brückner DB, Minchington TG, Greunz-Schindler M, Merrin J, Hannezo E, Kicheva A
Developmental cell 2025 Feb 24;60(4):567-580.e14

Cumulative experience meets modern science: Remarkable effects of TongXieYaoFang formula on facilitating intestinal mucosal healing and secretory function.

Du X, Liu L, Yang L, Zhang Y, Dong K, Li Y, Chen Y, Yang Q, Zhu X, Li Q
Journal of ethnopharmacology 2025 Feb 11;341:119370

Pharmacologic reversion of Merkel cell carcinoma via CBP/p300 inhibition.

Collura JL, Lei KC, Chandra M, Kervarrec T, Park HJ, Dalkoohi M, Becker JC, Chang Y, Moore PS, Shuda M
Proceedings of the National Academy of Sciences of the United States of America 2025 Dec 30;122(52):e2516667122

miR-219a-Robo1 axis acts as a conserved switch for slit-mediated commissural axon guidance.

Khot B, Suriyaarachchi H, Majumder T, Nathan A, Lu QR, Liu G
iScience 2025 Dec 19;28(12):114242

PEDV infection downregulates goblet cell differentiation through activating the Notch pathway.

Wang Y, Yang S, Zhao Y, Tian S, Cao Q, Geng X, Yang M, Song X, Shang H, Liu S, Guo R, Li Y, Sun M, Hu M, Fan B, Li B
Veterinary research 2025 Aug 12;56(1):168

Colonic epithelial-derived FGF1 drives intestinal stem cell commitment toward goblet cells to suppress inflammatory bowel disease.

Lin Q, Zhang S, Zhang J, Jin Y, Chen T, Lin R, Lv J, Xu W, Wu T, Tian S, Ying L, Li X, Huang Z, Niu J
Nature communications 2025 Apr 5;16(1):3264

Msx genes delineate a novel molecular map of the developing cerebellar neuroepithelium.

Gupta I, Yeung J, Rahimi-Balaei M, Wu SR, Goldowitz D
Frontiers in molecular neuroscience 2024;17:1356544

Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis.

Zheng Y, Li H, Qi S, Xiao F, Song J, Liu S, Chen X, Li Y, Chen M
Frontiers in pharmacology 2024;15:1534772

A primate model animal revealed the inter-species differences and similarities in the subtype specifications of the spiral ganglion neurons.

Hosoya M, Ueno M, Shimanuki MN, Nishiyama T, Oishi N, Ozawa H
Scientific reports 2024 Oct 24;14(1):25166

Actin Dysregulation Induces Neuroendocrine Plasticity and Immune Evasion: A Vulnerability of Small Cell Lung Cancer.

Seo Y, Zhang S, Jang J, Ko KP, Kim KB, Huang Y, Kim DW, Kim B, Zou G, Zhang J, Jun S, Chu W, Kirk NA, Hwang YE, Ban YH, Dhar SS, Chan JM, Lee MG, Rudin CM, Park KS, Park JI
bioRxiv : the preprint server for biology 2024 Nov 12;

Decoding the spatiotemporal regulation of transcription factors during human spinal cord development.

Shi Y, Huang L, Dong H, Yang M, Ding W, Zhou X, Lu T, Liu Z, Zhou X, Wang M, Zeng B, Sun Y, Zhong S, Wang B, Wang W, Yin C, Wang X, Wu Q
Cell research 2024 Mar;34(3):193-213

Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis.

Zhao J, Ma W, Wang S, Zhang K, Xiong Q, Li Y, Yu H, Du H
Redox biology 2024 Jun;72:103160

Notch1 is required to maintain supporting cell identity and vestibular function during maturation of the mammalian balance organs.

Heffer A, Lee C, Holt JC, Kiernan AE
bioRxiv : the preprint server for biology 2024 Jun 21;

Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells.

Luo Y, Wu K, Zhang X, Wang H, Wang Q
Stem cell research & therapy 2024 Jan 2;15(1):4

Protocol for fabricating elastomeric stencils for patterned stem cell differentiation.

Lehr S, Merrin J, Kulig M, Minchington TG, Kicheva A
STAR protocols 2024 Dec 20;5(4):103187

Epigenetic and Oncogenic Inhibitors Cooperatively Drive Differentiation and Kill KRAS-Mutant Colorectal Cancers.

Loi P, Schade AE, Rodriguez CL, Krishnan A, Perurena N, Nguyen VTM, Xu Y, Watanabe M, Davis RA, Gardner A, Pilla NF, Mattioli K, Popow O, Gunduz N, Lannagan TRM, Fitzgerald S, Sicinska ET, Lin JR, Tan W, Brais LK, Haigis KM, Giannakis M, Ng K, Santagata S, Helin K, Sansom OJ, Cichowski K
Cancer discovery 2024 Dec 2;14(12):2430-2449

A human fetal cerebellar map of the late second trimester reveals developmental molecular characteristics and abnormality in trisomy 21.

Yu H, Liu Y, Xu F, Fu Y, Yang M, Ding L, Wu Y, Tang F, Qiao J, Wen L
Cell reports 2024 Aug 27;43(8):114586

Early rhombic lip Protogenin(+ve) stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma.

Visvanathan A, Saulnier O, Chen C, Haldipur P, Orisme W, Delaidelli A, Shin S, Millman J, Bryant A, Abeysundara N, Wu X, Hendrikse LD, Patil V, Bashardanesh Z, Golser J, Livingston BG, Nakashima T, Funakoshi Y, Ong W, Rasnitsyn A, Aldinger KA, Richman CM, Van Ommeren R, Lee JJY, Ly M, Vladoiu MC, Kharas K, Balin P, Erickson AW, Fong V, Zhang J, Suárez RA, Wang H, Huang N, Pallota JG, Douglas T, Haapasalo J, Razavi F, Silvestri E, Sirbu O, Worme S, Kameda-Smith MM, Wu X, Daniels C, MichaelRaj AK, Bhaduri A, Schramek D, Suzuki H, Garzia L, Ahmed N, Kleinman CL, Stein LD, Dirks P, Dunham C, Jabado N, Rich JN, Li W, Sorensen PH, Wechsler-Reya RJ, Weiss WA, Millen KJ, Ellison DW, Dimitrov DS, Taylor MD
Cell 2024 Aug 22;187(17):4733-4750.e26

Mapping of Notch signaling in the developing organ of Corti in common marmosets.

Hosoya M, Fujioka M, Okano H, Ozawa H
Frontiers in neuroanatomy 2023;17:1188886

LSR targets YAP to modulate intestinal Paneth cell differentiation.

An Y, Wang C, Fan B, Wang Z, Li Y, Kong F, Zhou C, Cao Z, Wang M, Sun H, Zhao S, Gong Y
Cell reports 2023 Sep 26;42(9):113118

Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway.

Fang YX, Liu YQ, Hu YM, Yang YY, Zhang DJ, Jiang CH, Wang JH, Zhang J
Journal of ethnopharmacology 2023 May 23;308:116258

YAP1 and WWTR1 expression inversely correlates with neuroendocrine markers in Merkel cell carcinoma.

Frost TC, Gartin AK, Liu M, Cheng J, Dharaneeswaran H, Keskin DB, Wu CJ, Giobbie-Hurder A, Thakuria M, DeCaprio JA
The Journal of clinical investigation 2023 Mar 1;133(5)

Absence of gut microbiota impairs depletion of Paneth cells but not goblet cells in germ-free Atoh1(lox/lox) VilCreER(T2) mice.

Hassan M, Juanola O, Huber S, Kellmann P, Zimmermann J, Lazzarini E, Ganal-Vonarburg SC, Gomez de Agüero M, Moghadamrad S
American journal of physiology. Gastrointestinal and liver physiology 2023 Jun 1;324(6):G426-G437

Aryl Hydrocarbon Receptor Activation Coordinates Mouse Small Intestinal Epithelial Cell Programming.

Zhou X, Chakraborty D, Murray IA, Coslo D, Kehs Z, Vijay A, Ton C, Desai D, Amin SG, Patterson AD, Perdew GH
Laboratory investigation; a journal of technical methods and pathology 2023 Feb;103(2):100012

Rational incorporation of any unnatural amino acid into proteins by machine learning on existing experimental proofs.

Zhang H, Zheng Z, Dong L, Shi N, Yang Y, Chen H, Shen Y, Xia Q
Computational and structural biotechnology journal 2022;20:4930-4941

The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse.

Wu JPH, Yeung J, Rahimi-Balaei M, Wu SR, Zoghbi H, Goldowitz D
Frontiers in molecular neuroscience 2022;15:921901

Merkel Cell Carcinoma Sensitivity to EZH2 Inhibition Is Mediated by SIX1 Derepression.

Gartin AK, Frost TC, Cushman CH, Leeper BA, Gokhale PC, DeCaprio JA
The Journal of investigative dermatology 2022 Oct;142(10):2783-2792.e15

Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation.

Qin T, Ho CC, Wang B, Hui CC, Sham MH
Proceedings of the National Academy of Sciences of the United States of America 2022 Oct 25;119(43):e2206571119

Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals.

Iyer AA, Hosamani I, Nguyen JD, Cai T, Singh S, McGovern MM, Beyer L, Zhang H, Jen HI, Yousaf R, Birol O, Sun JJ, Ray RS, Raphael Y, Segil N, Groves AK
eLife 2022 Nov 29;11

Early development of the cochlea of the common marmoset, a non-human primate model.

Hosoya M, Fujioka M, Okahara J, Yoshimatsu S, Okano H, Ozawa H
Neural development 2022 May 7;17(1):6

Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment.

Morimoto Y, Fushimi A, Yamashita N, Hagiwara M, Bhattacharya A, Cheng J, Frost TC, Ahmad R, Daimon T, Huang L, Hata T, Takahashi H, Yamamoto M, Suzuki Y, DeCaprio JA, Kufe D
Oncogene 2022 Jul;41(27):3511-3523

Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases.

Brás J, Henriques D, Moreira R, Santana MM, Silva-Pedrosa R, Adão D, Braz S, Álvaro AR, de Almeida LP, Mendonça LS
Scientific reports 2022 Jul 22;12(1):12513

IL-17RA-signaling in Lgr5(+) intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.

Lin X, Gaudino SJ, Jang KK, Bahadur T, Singh A, Banerjee A, Beaupre M, Chu T, Wong HT, Kim CK, Kempen C, Axelrad J, Huang H, Khalid S, Shah V, Eskiocak O, Parks OB, Berisha A, McAleer JP, Good M, Hoshino M, Blumberg R, Bialkowska AB, Gaffen SL, Kolls JK, Yang VW, Beyaz S, Cadwell K, Kumar P
Immunity 2022 Feb 8;55(2):237-253.e8

CRISPR/Cas9-mediated genetic correction reverses spinocerebellar ataxia 3 disease-associated phenotypes in differentiated cerebellar neurons.

Song G, Ma Y, Gao X, Zhang X, Zhang F, Tian C, Hou J, Liu Z, Zhao Z, Tian Y
Life medicine 2022 Aug;1(1):27-44

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Fang J, Zhang Z, Cheng Y, Yang H, Zhang H, Xue Z, Lu S, Dong Y, Song C, Zhang X, Zhou Y
Food & function 2022 Apr 20;13(8):4399-4420

Efficient stimulation of retinal regeneration from Müller glia in adult mice using combinations of proneural bHLH transcription factors.

Todd L, Hooper MJ, Haugan AK, Finkbeiner C, Jorstad N, Radulovich N, Wong CK, Donaldson PC, Jenkins W, Chen Q, Rieke F, Reh TA
Cell reports 2021 Oct 19;37(3):109857

Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

Wilkerson BA, Zebroski HL, Finkbeiner CR, Chitsazan AD, Beach KE, Sen N, Zhang RC, Bermingham-McDonogh O
eLife 2021 May 18;10

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Jenkins BR, Blaseg NA, Grifka-Walk HM, Deuling B, Swain SD, Campbell EL, Walk ST, Kominsky DJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jun;35(6):e21551

Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea.

Billings SE, Myers NM, Quiruz L, Cheng AG
Development (Cambridge, England) 2021 Jun 1;148(11)

Intestinal MYC modulates obesity-related metabolic dysfunction.

Luo Y, Yang S, Wu X, Takahashi S, Sun L, Cai J, Krausz KW, Guo X, Dias HB, Gavrilova O, Xie C, Jiang C, Liu W, Gonzalez FJ
Nature metabolism 2021 Jul;3(7):923-939

Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice.

Wu H, Chen QY, Wang WZ, Chu S, Liu XX, Liu YJ, Tan C, Zhu F, Deng SJ, Dong YL, Yu T, Gao F, He HX, Leng XY, Fan H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jan;133:110937

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.

Park DE, Cheng J, McGrath JP, Lim MY, Cushman C, Swanson SK, Tillgren ML, Paulo JA, Gokhale PC, Florens L, Washburn MP, Trojer P, DeCaprio JA
Nature cell biology 2020 May;22(5):603-615

Merkel Cell Polyomavirus T Antigens Induce Merkel Cell-Like Differentiation in GLI1-Expressing Epithelial Cells.

Kervarrec T, Samimi M, Hesbacher S, Berthon P, Wobser M, Sallot A, Sarma B, Schweinitzer S, Gandon T, Destrieux C, Pasqualin C, Guyétant S, Touzé A, Houben R, Schrama D
Cancers 2020 Jul 21;12(7)

Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System.

Mattei C, Lim R, Drury H, Nasr B, Li Z, Tadros MA, D'Abaco GM, Stok KS, Nayagam BA, Dottori M
Frontiers in cell and developmental biology 2019;7:25

Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System.

Sayyid ZN, Wang T, Chen L, Jones SM, Cheng AG
Cell reports 2019 Jul 9;28(2):312-324.e4

Dual Regulatory Functions of SUFU and Targetome of GLI2 in SHH Subgroup Medulloblastoma.

Yin WC, Satkunendran T, Mo R, Morrissy S, Zhang X, Huang ES, Uusküla-Reimand L, Hou H, Son JE, Liu W, Liu YC, Zhang J, Parker J, Wang X, Farooq H, Selvadurai H, Chen X, Ngan ES, Cheng SY, Dirks PB, Angers S, Wilson MD, Taylor MD, Hui CC
Developmental cell 2019 Jan 28;48(2):167-183.e5

SCF/c-KIT Signaling Increased Mucin2 Production by Maintaining Atoh1 Expression in Mucinous Colorectal Adenocarcinoma.

Shen P, Yang S, Sun H, Li G, Wu B, Ji F, Sun T, Zhou D
International journal of molecular sciences 2018 May 22;19(5)

Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.

Atkinson PJ, Dong Y, Gu S, Liu W, Najarro EH, Udagawa T, Cheng AG
The Journal of clinical investigation 2018 Apr 2;128(4):1641-1656

Wls expression in the rhombic lip orchestrates the embryonic development of the mouse cerebellum.

Yeung J, Goldowitz D
Neuroscience 2017 Jun 23;354:30-42

Stem cells expanded from the human embryonic hindbrain stably retain regional specification and high neurogenic potency.

Tailor J, Kittappa R, Leto K, Gates M, Borel M, Paulsen O, Spitzer S, Karadottir RT, Rossi F, Falk A, Smith A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Jul 24;33(30):12407-22

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The ATOH1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human ATOH1. This antibody recognizes human,mouse,rat antigen. The ATOH1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 21215-1-AP.pdf

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